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MetaMorph Inc linescan function
Linescan Function, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/linescan+function/linescan+function/pm38201668-100-13-15
Average 90 stars, based on 1 article reviews
linescan function - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Diffusion-based Assay:

Article Title: Facilitated Transport across Glycocalyceal Barriers in the Chick Chorioallantoic Membrane.
Article Snippet: .. To assess tracer diffusion from the original application site, we used the standard linescan function (MetaMorph) to measure color intensity values along a linear (35 mm × 6 mm) region of interest. ..

Construct:

Article Title: Divalent cations activate TRPV1 through promoting conformational change of the extracellular region
Article Snippet: .. From these two images, the total emission spectrum and the TMRM emission spectrum, respectively, were constructed using the linescan function in MetaMorph to determine the fluorescence intensity values along the wavelength axis. ..

Article Title: General Theory for Integrated Analysis of Growth, Gene, and Protein Expression in Biofilms
Article Snippet: .. Using the linescan function in Metamorph Version 7.7.0.0, intensity versus distance curves were constructed for three cross-sections of each micrograph. ..

Fluorescence:

Article Title: Divalent cations activate TRPV1 through promoting conformational change of the extracellular region
Article Snippet: .. From these two images, the total emission spectrum and the TMRM emission spectrum, respectively, were constructed using the linescan function in MetaMorph to determine the fluorescence intensity values along the wavelength axis. ..

Article Title: Early endosome motility spatially organizes polysome distribution
Article Snippet: .. To measure ribosome or ER distribution, images of Rpl25-GFP or GFP-HDEL fluorescence were analyzed using the linescan function in MetaMorph. ..

other:

Article Title: Temporal-Spatial Visualization of Endogenous Chromosome Rearrangements in Living Cells
Article Snippet: In , linescans were performed using the “linescan” function in MetaMorph and plotted in Prism 8 (Graphpad.com).

Purification:

Article Title: Localization of Burkholderia cepacia Complex Bacteria in Cystic Fibrosis Lungs and Interactions with Pseudomonas aeruginosa in Hypoxic Mucus
Article Snippet: .. Purified bovine cervical mucins were incubated with trypsin (Trp) or crude lysates from B. cenocepacia (BC-7) or B. multivorans (J-1), and the products were resolved by 1% agarose gel electrophoresis (A and B) or 4-to-20% gradient PAGE (C to E). (A) The agarose gel was vacuum blotted and probed with a mucin-specific antibody. (B) The intensity profile for each lane of panel A was determined by using the linescan function in MetaMorph. ..

Incubation:

Article Title: Localization of Burkholderia cepacia Complex Bacteria in Cystic Fibrosis Lungs and Interactions with Pseudomonas aeruginosa in Hypoxic Mucus
Article Snippet: .. Purified bovine cervical mucins were incubated with trypsin (Trp) or crude lysates from B. cenocepacia (BC-7) or B. multivorans (J-1), and the products were resolved by 1% agarose gel electrophoresis (A and B) or 4-to-20% gradient PAGE (C to E). (A) The agarose gel was vacuum blotted and probed with a mucin-specific antibody. (B) The intensity profile for each lane of panel A was determined by using the linescan function in MetaMorph. ..

Agarose Gel Electrophoresis:

Article Title: Localization of Burkholderia cepacia Complex Bacteria in Cystic Fibrosis Lungs and Interactions with Pseudomonas aeruginosa in Hypoxic Mucus
Article Snippet: .. Purified bovine cervical mucins were incubated with trypsin (Trp) or crude lysates from B. cenocepacia (BC-7) or B. multivorans (J-1), and the products were resolved by 1% agarose gel electrophoresis (A and B) or 4-to-20% gradient PAGE (C to E). (A) The agarose gel was vacuum blotted and probed with a mucin-specific antibody. (B) The intensity profile for each lane of panel A was determined by using the linescan function in MetaMorph. ..

Polyacrylamide Gel Electrophoresis:

Article Title: Localization of Burkholderia cepacia Complex Bacteria in Cystic Fibrosis Lungs and Interactions with Pseudomonas aeruginosa in Hypoxic Mucus
Article Snippet: .. Purified bovine cervical mucins were incubated with trypsin (Trp) or crude lysates from B. cenocepacia (BC-7) or B. multivorans (J-1), and the products were resolved by 1% agarose gel electrophoresis (A and B) or 4-to-20% gradient PAGE (C to E). (A) The agarose gel was vacuum blotted and probed with a mucin-specific antibody. (B) The intensity profile for each lane of panel A was determined by using the linescan function in MetaMorph. ..



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GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) <t>Linescan</t> measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.
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GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) <t>Linescan</t> measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.
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GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) <t>Linescan</t> measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.
Linescan Function On Metamorph Software V7.7.8.0, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) Linescan measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.

Journal: Frontiers in Synaptic Neuroscience

Article Title: Locally-Induced CaMKII Translocation Requires Nucleotide Binding

doi: 10.3389/fnsyn.2020.00004

Figure Lengend Snippet: GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) Linescan measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.

Article Snippet: The density of GFP-CaMKII puncta was calculated by dividing the number of identified puncta by the length of the dendrite measured using the MetaMorph linescan function yielding a value in puncta/μm.

Techniques: Expressing, Translocation Assay, Transferring, Fluorescence